- Fellowship in 1998 at the University of Dundee
Dr. Yun Wah Lam received his PhD training in the lab of Dr. Davina Opstelten at the University of Hong Kong. After receiving his PhD, he joined the group of Prof. Angus Lamond in Dundee Scotland in 1998, where he developed an interest in the relationship of the architecture of mammalian cell nucleus and the regulation of gene expression. Lam uses live-cell imaging techniques and classical biochemical approaches to study protein localization and interactions in the cell nucleus. In parallel, he is involved in an international effort to characterise the human nucleolus proteome. In 2007, he joined the Department of Biology and Chemistry, City University of Hong Kong. His multi-disciplinary team uses quantitative mass spectrometry to measure the flux of proteins through subcellular spaces and to track the proteomic changes within cellular complexes through time. In complement, they record these dynamic cellular behaviours in live cells using time-lapse fluorescence microscopy. From these two angles, they aim at building coherent pictures of intracellular events under different growth and metabolic conditions.
Projects that have emerged from his lab in recent years include the characterization of protein flux through the nucleolus, the plasma membrane, the mitochondrion, and the extracellular matrix under various physiological conditions. To explore the potential of proteomics as a tool to reflect the dynamics of gene expression, they have also used pulse metabolic labelling of proteins with isotopic or functionally tagged amino acids.