Lui Chi Hang Vincent

  • Scholarship in 1989 at the The University of Hong Kong

Vincent Lui is a developmental molecular biologist.

 Research Interests:

  1. Molecular mechanisms controlling neural crest cells and enteric nervous system (ENS) development
  2. Genetics of neural crest cell associated developmental anomalies in human such as Hirschsprung's disease
  3. Molecular signaling mechanisms in urorectal development and their implications in human anorectal malformations
  4. Stem cells biology

Currently

Ongoing Projects:
We are now focusing in three important signaling pathways in the ENS development including the RET-HOXB5-TTF-1; and Hedgehog pathways. In addition, we also investigate the role of a number of homeobox containing genes in the ENS development, in particular, the HOXB5, Wnt and Hedgehog signaling pathways in cloaca septation and in human anorectal malformations. Multiple approaches are taken in my laboratory including (i) gene knockout in mice; (ii) transgenic mouse; (iii) ex vivo embryonic gut explant culture; (iv) in vitro NCC culture; (v) ex vivo culture of mouse genital tubercle; and (v) teratogen-induced developmental anomalies in rats and mice. I hope to develop and be able to apply NCCs as a stem cell replacement therapy for the treatment of HSCR and/or other NCC associated developmental anomalies.


Selected Publications:

  1. Fu M, Lui VCH, Sham MH, Pachnis V, Tam PKH. Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut. J Cell Biol 2004; 166(5): 673-684. 
  2. Garcia-Barcelo MM, Ganster RW, Lui VCH, Leon TYY, So MT, Lau AMF, Fu M, Sham MH, Knight J, Zannini MS, Sham PC, Tam PKH. TTF-1 and RET promoter SNPs: regulation of RET transcription in Hirschsprung's disease. Hum Mol Genet 2005; 14: 191-204.
  3. Garcia-Barcelo MM*, Miao X*, Lui VCH*, So MT, Ngan ES, Leon TY, Lau DK, Liu TT, Lao X, Guo W, Holden WT, Moore J, Tam PKH. Correlation between genetic variations in Hox clusters and Hirschsprung's disease. Ann Hum Genet 2007 Jul; 71(Pt 4): 526-536. (* These authors contributed equally to the work)
  4. Lui VCH, Cheng WWC, Leon TYY, Lau DKC, Garcia-Barcelo MM, Miao XP, Kam MKM, So MT, Chen Y, Wall NA, Sham MH, Tam PKH. Perturbation of Hoxb5 signaling in vagal neural crest cells down-regulates Ret leading to intestinal hypoganglionosis in mice. Gastroenterology 2008 Apr; 134: 1104-1115.
  5. Garcia-Barcelo MM*, Lui VCH*, Miao XP, So MT, Leon TYY, Yuan ZW, Li L, Liu L, Wang B, Sun XB, Huang LM, Tou JF, Ngan ESW, Cherny SS, Chan EKW, Lee KH, Wang W, Wong KKY, Tam PKH. Mutational analysis of SHH and GLI3 in anorectal malformations. Birth Defects Res A Clin Mol Teratol 2008 Sep; 82(9): 644-648. (* These authors contributed equally to the work)
  6. Garcia-Barcelo MM, Tang CS, Ngan ESW, Lui VCH, Chen Y, So MT, Leon TY, Miao XP, Shum CK, Liu FQ, Yeung MY, Yuan ZW, Guo WH, Liu L, Sun XB, Huang LM, Tou JF, Song YQ, Chan D, Cheung KM, Wong KK, Cherny SS, Sham PC, Tam PKH. Genome-wide association study identifies NRG1 as a susceptibility locus for Hirschsprung's disease. Proc Natl Acad Sci U S A 2009 Feb 24; 106(8): 2694-2699. 
  7. Zhu, J., Garcia-Barceló, M.M., Tam, P.K., Lui, V.C.H. (2011) HOXB5 Cooperates with NKX2-1 in the Transcription of Human RET. PLoS One. June 3;6(6):e20815.
  8. Ngan, E.S.W., Garcia-Barcelo, M.M., Yip, B.H.K., Poon, P.C., Lau, C.S.T., Kwok, K.M., Sat, E.W.Y., Sham, M.H., Wong, K.K.Y., Wainwright, B.J., Cherny, S.S., Hui, C.C., Sham, P.C., Lui, V.C.H., Tam, P.K.H. (2011) Hedgehog/Notch-induced premature gliogenesis represents a new disease mechanism for Hirschsprung’s disease in mice and humans.  J Clin Invest. 2011 Sep 1;121(9):3467-78.
  9. Li, H., Gao, F, Ma, L., Jiang, J., Miao, J., Jiang, M., Fan, Y., Wang, L., Wu, D., Liu, B., Wang, W., Lui, V.C., Yuan, Z. (2011) Therapeutic potential of in utero mesenchymal stem cell (MSCs) transplantation in rat fetuses with spina bifida aperta. J Cell Mol Med. 2011 Oct 18. doi: 10.1111/j.1582-4934.2011.01470.x. [Epub ahead of print] 
  10. So, M.T., Leon, T.Y.Y., Cheng, G., Tang, C.S.M., Miao, X.P., Cornes, B.K., Ngo, D.N., Cui, L., Ngan, E.S.W., Lui, V.C.H., Wu, X.Z., Wang, B., Wang, H., Yuan, Z.W., Huang, L.M., Li, L., Xia, H., Zhu, D., Liu, J., Nguyen, T.L., Chan, I.H.Y., Chung, P.H.Y., Liu, X.L., Zhang, R., Wong, K.K.Y., Sham, P.C., Cherny, S.S., Tam, P.K.H., Garcia-Barcelo, M.M. (2011) RET mutational spectrum in Hirschsprung disease: evaluation of 601 Chinese patients. PLoS ONE 6(12):e28986. Epub 2011 Dec 9
  11. Leon, T.Y.Y., So, M.T., Lui, V.C.H., Hofstra, R.M.W., Tam, P.K.H., Ngan, E.S.W., Garcia-Barcelo, M.M. (2012) Functional analyses of RET mutations in Chinese Hirschsprung disease patients. Birth Defects Research Part A: Clinical and Molecular Teratology. Jan;94(1):47-51. doi: 10.1002/bdra.22863. Epub 2011 Dec 1
  12. Kam, M.K.M., Lee, K.Y., Tam, P.K.H., Lui, V.C.H. (2012) Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. PLoS ONE 2012;7(5):e35799. Epub 2012 May 7 (I.F. 4.351)
  13. Tang, C.S., Cheng, G., So, M.T., Yip, B.H.K., Miao, X.P., Wong, E.H.M., Ngan, E.S., Lui, V.C.H., Song, Y.Q., Chan, D., Cheung, K., Yuan, Z.W., Lei, L., Chung, H.Y., Liu, X.L., Wong, K.K.Y., Marshall, C.R., Scherer, S., Cherny, S.S., Sham, P.C., Tam, P.K.H., Garcia-Barceló, M.M. (2012) Genome-wide copy number analysis uncovers a new HSCR gene: NRG3. PLoS Genetics (In Press)